GEVHER NESİBE 4. ULUSLARARASI SAĞLIK BİLİMLERİ KONGRESİ, Ankara, Turkey, 23 - 24 November 2019, vol.4, pp.222-224
The aim of this study was to evaluate the possible protective effects of atorvastatin (ATO) and cinnamon (Cin) treatments on rat testes in the experimental diabetes (DM) model at level of biochemical and histological by light microscope. Wistar albino adult rats were divided into 8 groups as Normal-Control (C), Citrate buffer-C, Cin-C, ATO-C, DM, DM+Cin, DM+ATO, DM+ATO+Cin. Streptozotocin (STZ) as a single dose (35 mg/kg) was administered to rats to form a DM model. For ten weeks, ATO was given to groups of ATO-C, DM+ATO, DM+ATO+Cin, and Cin juice was given to groups of Cin-C, DM+Cin, DM+ATO+Cin. At the end of this period, biochemical analysis of blood and testis was performed. Testis sections were examined histologically under light microscope. The data obtained were evaluated statistically (p <0.05).
There was a significant difference in blood glucose levels between the control and other groups (p <0.05). Lowest blood glucose level was found in DM+Cin group however it was not statistically significant. The lowest body weights were in the DM group. Testicular weights were lower in DM group compared to other groups, but difference was not significant. The lowest total cholesterol level was observed in the control group. The highest serum MDA levels were in the Cin-C and DM groups. A significant difference was found between the DM group and the other experimental groups, and ATO-C group and the other control groups in terms of testicular tissue MDA levels. The highest tissue MDA levels were determined in DM and ATO-C groups. There was a significant difference between ATO-C group and other groups according to tissue GSH levels (p <0.05). Light microscopically, seminiferous tubules with the lowest diameter were found in the DM group and the highest diameter tubules were found in the Cin-C group. According to Johsen score, the highest score was found in Normal-C and the lowest score was in DM group. The highest apoptotic index was found in the DM group and the lowest apoptotic index was found in the ATO-C group by TUNEL technique. When comparing the DM group and treatment groups, we concluded that the severity of diabetes-induced tissue damage could be reduced with ATO and Cinnamon treatment.
Keywords: Diabetes Mellitus, Cinnamon, Atorvastatin, Testis, Streptozotocin, Testosteron, Total Cholesterol, Total Oxidant Status, Total Antioxidant Status, Apoptosis