Akademik Veri Yönetim Sistemi
Acta Medica Nicomedia, cilt.6, sa.1, ss.131-136, 2023 (Hakemli Dergi)
Objective: The difficulties experienced in the treatment of prostate cancer and the excess of side effects due to chemotherapy have brought the search for alternative treatment strategies. In recent studies, it is known that Rutin (RUT) has an anti-cancer effect on cancer cells. Our study aimed to determine the effects of RUT on epithelial to mesenchymal transition (EMT) in prostate cancer cells, for the first time. Methods: The anticancer effect of RUT in prostate cancer cells (PC-3) was examined by WST-1, Annexin V ELISA, DAPI and Acridine Orange staining, and the anti-cancer and anti-metastatic properties of RUT were evaluated with the Scratch Assay test. The expression level of Bax, Bcl-2, Snail, Twist, Vimentin and Ecadherin genes was evaluated by RT-PCR. Results: The cells were treated with RUT (500, 750, 1000 and 1500 µM) for 24 and 48 hours. The viability rates decreased with increasing RUT concentration depending on dose and time (p<0.01). Free Annexin V protein decreased, and apoptotic cells increased depending on the increasing RUT concentration (p<0.01). There was a reduction in the migrating cells, especially at 750 and 1000 µM RUT concentrations. There was a significant increase (14.22-fold) in E-cadherin mRNA level after 1500 µM RUT treatment (p<0.01). Additionally, the mRNA level of Snail, Twist and Vimentin decreased at higher RUT concentrations (p<0.01). Conclusion: The existence of RUT's potential to inhibit epithelial to mesenchymal transition and promote apoptosis has been demonstrated. It is also recommended to explore the effects of RUT on EMT in vivo