A comparative study of the effects of hemin and bilirubin on bilateral renal ischemia reperfusion injury

Demirogullari B., Ekingen G., Guz G., Bukan N., Erdem O., Ozen I. O., ...More

NEPHRON EXPERIMENTAL NEPHROLOGY, vol.103, no.1, pp.1-5, 2006 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 103 Issue: 1
  • Publication Date: 2006
  • Doi Number: 10.1159/000090113
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1-5
  • Kocaeli University Affiliated: No


Background/Aims: The aim of this study was to determine the effects of hemin, a heme oxygenase-1 inducer, and bilirubin on renal ischemia-reperfusion (I-R) injury. Methods: 40 Wistar-Albino rats were allocated into six groups as follows: sham (S), bilirubin (B), hemin (H), ischemia/ reperfusion (IR), IR + bilirubin (IRB) and IR + hemin (IRH). Conjugated bilirubin (20 mg center dot kg(-1) i. v.) was given to rats in groups B and IRB, and hemin (50 mg center dot kg (-1) i. p.) was given to rats in groups H and IRH just prior to reperfusion. Renal I-R was achieved by occluding the renal arteries bilaterally for 50 min. Following 6 h of reperfusion, blood was drawn to study BUN, creatinine and bilirubin, and tissue samples were harvested to determine the renal malonyldialdehyde and heme oxygenase-1 levels, and for histopathologic grading. Results: BUN, creatinine and malonyldialdehyde levels in group IRH were similar to controls whereas the results of groups IR and IRB were signifi cantly higher (p < 0.01). There was a grade 2 damage in all I-R groups. Conclusion: This study showed the preventive effect of hemin on renal ischemia reperfusion injury. Administration of exogenous bilirubin did not prevent the I-R injury. (C) 2006 S. Karger AG, Basel.