Improvement in desulfurization of dibenzothiophene and dibenzothiophene sulfone by Paenibacillus strains using immobilization or nanoparticle coating

Sar T., Ozturk M., Stark B. C., Akbas M. Y.

Journal of Applied Microbiology, cilt.133, sa.2, ss.1040-1051, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 133 Sayı: 2
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1111/jam.15637
  • Dergi Adı: Journal of Applied Microbiology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database, DIALNET
  • Sayfa Sayıları: ss.1040-1051
  • Anahtar Kelimeler: desulfurization, dibenzothiophene, dibenzothiophene sulfone, immobilization, nanoparticle coating, Paenibacillus, BIODESULFURIZATION, IGTS8, CELLS, PATHWAY, BEADS
  • Kocaeli Üniversitesi Adresli: Evet

Özet

© 2022 Society for Applied Microbiology.Aims: Biodesulfurization of fossil fuels is a promising technology for deep desulfurization. Previously, we have shown that Paenibacillus strains 32O-W and 32O-Y can desulfurize dibenzothiophene (DBT) and DBT sulfone (DBTS) effectively. In this work, improvements in DBT and DBTS desulfurization by these strains were investigated through immobilization and nanoparticle coating of cells. Methods and Results: Paenibacillus strains 32O-W and 32O-Y immobilized in alginate gel beads or coated with Fe3O4 magnetite nanoparticles were grown at various concentrations (0.1–2 mmol l−1) of DBT or DBTS for 96 h. The production of 2-hydroxybiphenyl (2-HBP) from the 4S pathway biotransformation of DBT or DBTS was measured. The highest amounts of 2-HBP production occurred at concentrations of 0.1 and 0.5 mmol l−1. Compared to planktonic cultures maximum 2-HBP production increased by 54% for DBT and 90% for DBTS desulfurization with immobilized strains, and 44% for DBT and 66% for DBTS desulfurization by nanoparticle-coated strains. Conclusions: Nanoparticle-coated and immobilized cells may be of use in efforts to increase the efficiency of biodesulfurization. Significance and Impact of the Study: Alginate immobilization or nanoparticle coating of bacterial cells may be useful approaches for the enhancement of biodesulfurization for eventual use on an industrial scale.