Casein fibres for wound healing

Ahmed J., Guler E., Sinemcan Ozcan G., Emin Cam M., Homer-Vanniasinkam S., Edirisinghe M.

Journal of the Royal Society, Interface, vol.20, no.204, pp.20230166, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 204
  • Publication Date: 2023
  • Doi Number: 10.1098/rsif.2023.0166
  • Journal Name: Journal of the Royal Society, Interface
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Animal Behavior Abstracts, BIOSIS, CAB Abstracts, Compendex, INSPEC, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.20230166
  • Keywords: bandages, casein, fibres, in vivo, wound healing
  • Kocaeli University Affiliated: Yes


The name casein is given to a family of phosphoproteins which is commonly found in milk. Until recently, this was a constituent of milk that was commonly discarded; however today, it is widely used in health supplements all over the world. In this work, a high loading (50 wt%) of casein is mixed with a solution of polycaprolactone (PCL) to produce bandage-like fibres with an average fibre diameter of 1.4 ± 0.5 µm, which would be used to cover wounds in a series of tests with diabetic rats. Mouse fibroblast cell viability tests show that the casein-loaded fibres had little cytotoxicity with over 90% observed viability. A 14-day in vivo trial involving three groups of rats, used as control (no treatment), pure PCL fibres and casein-loaded fibres, showed that the casein within the fibres contributed to a significantly more extensive healing process. Histological analysis showed increased development of granulation tissue and follicle regrowth for the casein-loaded fibres. Further analysis showed that casein-loaded fibres have significantly lower levels of TNF-α, TGF-β IL-1β, NF-κB and IL-6, contributing to superior healing. The results presented here show an economical and simple approach to advanced wound healing.