Akademik Veri Yönetim Sistemi
Gazi Üniversitesi Fen Bilimleri Dergisi Part C: Tasarım ve Teknoloji, cilt.12, sa.1, ss.25-39, 2024 (Hakemli Dergi)
Ventilator-associated pneumonia (VAP) is one of the most important infections in the intensive care unit (ICU) and contributes to increased mortality and morbidity in patients. In this study, we aimed to evaluate Rheum ribes (Elazig/Turkey) extract on bacterial isolates obtained from VAP patients the antimicrobial effects (agar well diffusion, MIC test) and antioxidant capacity (DPPH, FRAP and metal chelation). As a result of the analysis, the highest antimicrobial effect of R.ribes was observed in Klebsiella pneumonia-2 (K.pneumonia) and K.pneumonia-21 isolates, with zone diameters of 19.32 and 18.45 mm, respectively. Apart from these, Staph. aureus., K. pneumonia- 20, K. pneumonia-16 and Pseudomonas aeruginosa (P. aeruginosa) were detected with zone diameters of 18.32, 15.14, 14.56 and 13.54 mm, respectively. R. ribes extract showed 98.3% and 94.88% inhibitory effect at 0.5 ppm in K.pneumonia isolates 16 and 20 while, it showed 100% inhibitory effect on the highest isolates 21 and 2. S. aureus showed a high inhibition effect of 50.36% at 0.25 ppm, and P. aeruginosa isolate at a rate of 82.82% at 0.5 ppm. Besides, DPPH, FRAP and metal chelation analyzes revealed a strong antioxidant effect. DPPH inhibition effect, FRAP analysis and chelating activity values of iron ions (Fe2+) for the antioxidant effects of R.ribes extract were determined as 17.22%, 1.18 and 2.14%, respectively. As a result, strong effect and antioxidant capacity of R. ribes extract on pathogenic bacteria have been determined, and its beneficial properties can be deepened by in vivo and clinical studies.