Akademik Veri Yönetim Sistemi
International Marmara Social Sciences Congress (Imascon Autumn 2022), Kocaeli, Türkiye, 9 - 10 Aralık 2022, ss.65, (Özet Bildiri)
Parasitoids are important biological control agents who have evolved different reproductive strategies depending on the host. Parasitoid venoms contain components that inhibit and paralyze host development and suppress the immune system. The venom of Pimpla turionellae, an idiobiont, solitary endoparasitoid, consists of a mixture of medium/high molecular weight proteins, various enzymes, peptides and paralytic factors. However, the oxidative effects of the venom on larval hemolymph have not been clarified. Therefore, the oxidative effects of different doses of P. turionellae venom on host Galleria mellonella were evaluated. Venom sacs of P. turionellae females were collected under stereoscopic microscope and various venom concentrations (0.005 VRE (venom reservoir equivalence), 0.01 VRE, 0.05 VRE, 0.1 VRE, 0.5 VRE) were prepared. Then they were injected into G. mellonella larvae. Malondialdehyde (MDA), advanced oxidation protein products (AOPP) and ferric antioxidant power (FRAP) were measured in larval hemolymph samples by spectrophotometer. Protein amounts were also determined using the Bishinkoninic acid method in spectrophotometry. MDA and AOPP levels decreased in hemolymph depending on P. turionellae venom, but no significant change was recorded in FRAP levels. However, it was observed that the reduction power of iron increased at 0.1 VRE concentration of the venom. According to the results obtained, it was determined that the parasitoid venom suppressed or reduced oxidative stress. It is also thought that the venom has antioxidant features and can be applied in alternative therapeutic methods.