Akademik Veri Yönetim Sistemi
Enzyme and Bioprcess Days, EBDAY'S 2024, İzmir, Türkiye, 27 - 29 Ağustos 2024, ss.45, (Özet Bildiri)
Proteases are valuable enzymes that have important
applications in industry. Among various industrial enzymes, particularly
microbial proteases dominate the world enzyme market due to their versatile
application potential in bioindustry. Currently, with a market exceeding $2
billion annually, such alkaline stable hydrolases have applications in
detergents, food additives, and biomass degradation. Numerous investigations
have been carried out on alkaline proteases from strains that produce high
yields, but extreme highly solvent-stable enzymes are rarely found, excluding
limited studies. In this work, reported that solvent stability properties of
alkaline protease produced by extreme obligate alkaliphile Bacillus marmarensis
strain DSM-21297. The influence of several mono-alcohols was examined on the
protease stability at concentrations of 10-60%(v/v) after
2h pre-incubation at 30°C. Surprisingly,
the protease activity was marginally enhanced in the presence of methanol and
ethanol. Both solvents had similar effects on DSM-21297 protease activity.
Enzyme activity increased with increasing solvent concentrations. After 2h
pre-incubation at 30°C in the presence of methanol (10-40%)
and ethanol (10-30%) activity elevated 152% and 165%, respectively. A similar
result was also observed in stability after 2h of pre-incubation in the
presence of 30% methanol and ethanol at 45°C. Enzyme maintained its stability
by 100%. The enzyme was found to be significantly more stable in organic
solvents compared to other microbial proteases from Bacillus sp. As a result, the most useful application of DSM-21297
protease, with its solvent-resistant nature, is the synthesis of peptides and
esters in non-aqueous environments in industries. This
is the first study on the solvent stability of B. marmarensis protease.