Practical approach for detection and identification of OXA-10-derived ceftazidime-hydrolyzing extended-spectrum beta-lactamases

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Vahaboglu H., Ozturk R., Akbal H., Saribas S., Tansel O., Coskunkan F.

JOURNAL OF CLINICAL MICROBIOLOGY, vol.36, no.3, pp.827-829, 1998 (SCI-Expanded) identifier identifier identifier

Abstract

A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 beta-lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of bla(OXA) was amplified by PCR from the hybridization-positive isolates, and the products were digested by PvuII and HaeIII.