Akademik Veri Yönetim Sistemi
45th FEBS Congress, Molecules of Life: Towards New Horizons, Ljubljana, Slovenia, July 3–8, 2021, Ljubljana, Slovenya, 3 - 08 Temmuz 2021, cilt.11, sa.1, ss.487, (Özet Bildiri)
P-08.5-30
Assessment of melatonin effect on redox balance and biochemical changes in chronically cigarette smoke-exposed rat liver tissue
F. Huncß1 , E. Kole2 , H. Maral Kır 3 , E. Acar3 , M. Dillioglugil3 , S. Ozdemir Ozkan4
1Kocaeli University, Faculty of Medicine, Departmant of Medical Biochemistry, Kocaeli, Turkey,
2Darica Farabi Training and Research Hospital, Department of Obstetrics and Gynecology, Kocaeli, Turkey,
3Kocaeli University, Faculty of Medicine, Department of Biochemistry, Kocaeli, Turkey, Kocaeli, Turkey,
4 Kocaeli Medical Park Hospital, Obstetrics and Gynecology Clinic, Kocaeli, Turkey
Cigarette smoke is one of the exceedingly widespread hazardous attitude along with its complex mixture of toxicants, causes cellular damage by generating free radicals. Smoke exposure enhances oxidative stress and diminishes antioxidant defence mechanisms. Particularly in recent studies are focused on its toxic effects on visceral organs alongside its major impact on the respiratory system. Melatonin, an indolic hormone derived from the amino acid tryptophan, acts as strong antioxidant and free radical scavenger and also has anti-inflammatory, and antioxidant properties. This research was carried out to examine the effects of the subcutaneous melatonin administration on oxidative liver damage caused by chronic cigarette smoke-exposure in an experimental rat model. Forty adult female Wistar-Albino rats were used as experiimental subjects. The rats were randomized and divided into 4 groups including a nonsmoking control group (C), control of subcutaneous 20 mg/kg melatonin (CM) administration, long-term cigarette smoke exposed group (SE), and SE with subcutaneous 20 mg/kg melatonin administration (SM), each containing 10 rats. Chronical cigarette smoke exposure lasted 45 days of experimental period of time. Biochemical analysis was conducted on rat liver tissue homogenates. MDA, NO, AOPP, GSH, nuclear factor kappa B (NF-jB), and thioredoxin interacting protein (TXNIP) levels were measured. In this research, biomarkers, related to the cellular damage and oxidative stress were found higher (MDA, NO, AOPP, NF-jB, and TXNIP) in the SE group, whereas GSH was depleted (P < 0.05). The most noticeable result of melatonin treatment was found in the CM group with significant decrease of MDA and GSH levels. Cigarette smoking due to its cellular damage, deplete antioxidants and also increases oxidative stress, consequently results in inflammatory response. Conversely to cigarette smoke cellular toxicity, melatonin treatment may have regulatory effects on rat liver tissue.