Investigation of compatibility of severe acute respiratory syndrome coronavirus 2 reverse transcriptase-PCR kits containing different gene targets during coronavirus disease 2019 pandemic


Sarigul F., Doluca O., AKHAN S. , SAYAN M.

FUTURE VIROLOGY, vol.15, no.8, pp.515-524, 2020 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 15 Issue: 8
  • Publication Date: 2020
  • Doi Number: 10.2217/fvl-2020-0169
  • Title of Journal : FUTURE VIROLOGY
  • Page Numbers: pp.515-524

Abstract

Aim:In the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), reverse transcriptase-PCR (RT-PCR) technique is often used. We evaluated the compatibility of SARS-CoV-2 RT-PCR kits containing different gene targets during the pandemic.Materials & methods:Samples were tested by Bio-Speddy(R)(RdRpgene) and Diagnovital(R)(RdRp + Egenes). The correlation between two assays were determined by Deming regression analysis and chi-square analyses.Results:Diagnovital PCR kit showed amplification in a narrow Ct range and conveniently sharper exponential amplification curves than Bio-Speedy PCR kit. While the correlation between the findings of the two kits was apparent even with single gene target, this correlation increased when a secondary biomarker was added to the correlation calculations.Conclusion:We have observed high correlation between different PCR kits, however, using different PCR kits during the pandemic may provide a more accurate diagnosis of SARS-CoV-2, since despite correlation there are a number of patients showing contradicting diagnosis.