Determination of Naphthazarin Derivatives in 16 Alkanna Species by RP-LC Using UV and MS for Detection

Akgun I., Ganzera M., Gur C. , Senol S., Korkmaz K., BEDİR E.

CHROMATOGRAPHIA, cilt.70, ss.963-967, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 70
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1365/s10337-009-1261-2
  • Sayfa Sayıları: ss.963-967


As part of our ongoing studies, a new and validated method for analysis of isohexenyl-naphthazarines in Turkish Alkanna species was developed. By using reversed phase material (Synergi MAX RP) it was possible to determine four known isohexenylnaphthazarin-derivatives, namely alkannin, acetylalkannin, deoxyalkannin, and beta,beta-dimethylacrylalkannin within 25 min in n-hexane extracts of the roots. The quantification of these compounds in 16 Anatolian Alkanna species is described for the first time. In order to assure peak identity LC-MS experiments were performed. Quantitative results revealed that the most dominant compound in the extracts was beta,beta-dimethylacrylalkannin.