Investigation of the Antibacterial Effect of Taraxacum officinale Extracts Extracted in Different Solvents on Bacteroides fragilis ATCC 25285 Standard Strain by Broth Microdilution Method Farklı Çözücülerde Ekstrakte Edilen Taraxacum officinale Ekstraktlarının Bacteroides fragilis ATCC 25285 Standart Suşu Üzerindeki Antibakteriyel Etkisinin Sıvı Mikrodilüsyon Yöntemiyle Araştırılması


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ALTAN G., BUDAK F.

Mikrobiyoloji bulteni, cilt.58, sa.1, ss.63-70, 2024 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58 Sayı: 1
  • Basım Tarihi: 2024
  • Doi Numarası: 10.5578/mb.20249906
  • Dergi Adı: Mikrobiyoloji bulteni
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.63-70
  • Kocaeli Üniversitesi Adresli: Evet

Özet

Bacteria causing hospital-associated infections continue to become resistant due to antibiotic resistance, which has become a global problem worldwide and accordingly, the antibiotic options used in the treatment of infectious diseases caused by these bacteria are limited. In the light of the data obtained from experimental studies on plants, it is thought that plant extracts may be a promising option in the treatment of infectious diseases. In this study, it was aimed to determine the antibacterial activity of Taraxacum officinale extracts on Bacteroides fragilis ATCC 25285 standard strain by broth microdilution method and to pioneer different studies that will investigate the antibacterial effects of plant extracts on resistant B.fragilis strains that cause hospital-acquired opportunistic infections after invasive interventions and trauma. In this study, the T.officinale plant collected as a result of field work was divided into root, leaf and flower parts and dried at 70 °C for 24 hours and then turned into powder. Dried plant samples were extracted in ethanol and methanol for 24 hours. The obtained extracts were stored at -80 °C to be used in the broth microdilution method. B.fragilis ATCC 25285 standard strain was used as the bacterial strain. As a result of the experiments performed with broth microdilution method, the MIC (minimum inhibitory concentration) value of root, leaf and flower extracts with ethanol was determined as 200 μg/ mL, the methanolic root extract as 100 μg/mL and the methanolic leaf and flower extracts as 200 μg/mL. As a result, ethanol and methanol plant extracts were found to be effective on B.fragilis strain.