Preliminary screening the antioxidant potential of in vitro-propagated Amsonia orientalis: An example to sustainable use of rare medicinal plants in pharmaceutical studies

Kıran Acemi R., Acemi A., Çakır M., Gün Polat E., Özen F.

SUSTAINABLE CHEMISTRY AND PHARMACY, vol.17, pp.1-7, 2020 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 17
  • Publication Date: 2020
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1-7
  • Kocaeli University Affiliated: Yes


Amsonia orientalis (European Bluestar) is a critically endangered plant species with medicinal and ornamental

properties. The rare availability of the species in nature limits its potential to be used for various purposes.

However, plant tissue culture is an effective method for the cultivation of such vulnerable species without

damaging their natural populations, which are very limited in nature for scientific purposes. By taking advantage

of plant tissue culture, this study aimed to measure the phenolic substance and flavonoid contents in leaf extracts

of in vitro-propagated Amsonia orientalis, and to investigate their antioxidant potentials through phosphomolybdate

and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The crude extracts prepared in

water, aqueous ethanol, methanol, and acetone were tested. The highest phenolic substance content was found in

the ethanolic extracts, while statistically the same flavonoid contents were found in the ethanolic, methanolic,

and acetone extracts. Although the water extract had lesser flavonoid content, it exhibited a notable antioxidant

property. The ethanolic leaf extract gave the highest antioxidant and DPPH radical scavenging activity, especially

when used at 1 mg mL􀀀 1 concentration. Also, the TLC fingerprint profile validated the presence of valuable

phytoconstituents in the leaves of the plant. This study indicated that ultrasound-assisted extraction of minimal

amounts of dried leaf samples from in vitro-propagated plants might be adequate for the pre-screening of the

antioxidant capacity of rare plant species.