In this study, it is aimed to investigate the physical properties of poly(lactic acid)/thermoplastic starch (PLA/TPS) blends as a function of number of recycling (NOR) for different blend compositions. The flexural and impact properties, dynamic mechanical behavior, thermal transitions, thermal stability, phase, and fracture morphology are presented. Thermogravimetric analysis shows that the thermal stability of blends diminished by the increasing amount of TPS. It is revealed from differential scanning calorimetry results that the glass transition temperature (T-g) and melting temperature (T-m) are negatively affected by the NOR. Flexural and impact tests show that the mechanical properties are deteriorated by NOR. The TPS phase size becomes coarser with NOR as observed from scanning electron microscopy. It is concluded that the properties of PLA/TPS blends are very sensitive to NOR and the PLA/TPS concentration as well.