Assessment of FTO Protein Expression Levels in Various Cancer Cell Lines by Western Blotting


Kanlı A., Yanar S.

3rd International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2020), Ankara, Turkey, 19 - 20 March 2020, pp.467-476

  • Publication Type: Conference Paper / Full Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.467-476
  • Kocaeli University Affiliated: Yes

Abstract

Assessment of FTO Protein Expression Levels in Various Cancer Cell Lines by Western Blotting 
 
Abstract
 
Fat-mass and obesity-associated (FTO) protein is a member of the alpha-ketoglutarate dependent hydroxylase family and has demethylase activity, genome-wide association studies (GWAS), carried out since 2007, have shown that intronic FTO gene polymorphisms are closely related to obesity and increased body mass index. In addition, many recent studies have shown an association between the FTO and various diseases e.g., cardiological and neurological diseases and various types of cancers. FTO polymorphisms are found to be closely associated with many types of cancers. FTO overexpression is proposed to contribute to the growth, self-renewal and metastasis of these cancer. However, the mechanisms by which this protein play roles in cancer formation and progression are still not fully understood. The studies conducted are mostly at the mRNA level and there are no studies investigating the association between the FTO and cancer at the proteomic level. In this study, we aimed to analyze of the FTO protein expression levels in different cancer lines and their healthy counterparts by western blotting. The data produced from this study can be used to generate comprehensive TÜBİTAK or BAP projects, in which we will investigate the relationship and mechanism of FTO with cancer by proteomic methods. We observed that FTO protein expression in breast cancer cell line MDA-MB-231, lung cancer cell line DMS114 and prostate cancer cell lines DU145 and 22RV were more than the healthy counterparts. This simple observation is satisfactory to directly associate FTO with various cancers.  This study also demonstrated that cancer cell lines are good models for elucidation of multifunctional roles of FTO. Tools silencing FTO in cancer cell lines can be used in comparative transcriptomic, metabolomics and proteomics studies.   
 
Key words: FTO protein, Cancer cell lines, Western Blotting