Akademik Veri Yönetim Sistemi
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, cilt.39, sa.3, ss.145-161, 2024 (SCI-Expanded)
Gelatin is a collagen-derived biopolymer with high biocompatibility, commonly used in the production of wound dressing materials. Gelatin-based wound dressings, obtained through different processing methods, come with their own advantages and disadvantages depending on their physical structure. In this study, the effects of gelatin wound dressing materials with two different morphologies on wound healing are investigated in detail. The study investigated that the preparation of gelatin-based wound dressing materials using two different methods, followed by their chemical characterization and examination of their effects on biocompatibility and wound healing. To comprehend the impact of nanofiber structure and porous sponge structure on the wound healing process, the chemical structure of the prepared wound dressings was analyzed using Fourier Transform Infrared Spectrophotometer (FTIR). The morphological structure was visualized through Scanning Electron Microscope (SEM), the thermal properties were determined using Thermogravimetric Analyzer (TGA) and Differential Scanning Calorimeter (DSC). Additionally, the in vivo wound healing effects of gelatin-based wound dressings with different structures were evaluated through macroscopic observation and histological analysis in a dorsal region wound-healing rat model, monitored for 21 days. It was observed that wound healing materials with desired nanofiber and porous sponge structures were successfully obtained based on the SEM analysis. The gelatin nanofiber dressing showed a randomly oriented, bead-free, and smooth morphology with an average fiber diameter of approximately 1250 +/- 0.35 nm, while the crosslinked gelatin sponge dressing had pore sizes ranging from 20 to 160 mu m, and interconnected pore structures. The chemical structure remained unaltered according to FTIR graphs and these results were further supported by DSC and TGA graphs. Results from in vitro biocompatibility studies indicated that the wound dressing materials were biologically compatible, non-toxic, and did not cause irritation or sensitivity to the skin. Regarding in vivo analysis, it was determined that gelatin dressing materials were functional in the wound healing process, without the use of any auxiliary materials. Graphical Abstract This is a visual representation of the abstract.