Akademik Veri Yönetim Sistemi
INTERNATIONAL JOURNAL OF CHEMICAL KINETICS, 2026 (SCI-Expanded, Scopus)
We report a systematic investigation of solvent-induced modulation of Bacillus clausii protease catalysis, focusing on correlations between kinetic and thermodynamic parameters and solvent descriptors (log P, dielectric constant, pi*, beta, ETN). Our findings demonstrate that the effect of DMSO on protease catalysis exhibited a dual behavior, with activity enhancement observed at lower solvent concentrations (up to 20% v/v), as revealed by activity measurements, while kinetic analyses performed at higher DMSO concentrations (>= 30% v/v) indicated simple uncompetitive inhibition, as evidenced by Lineweaver-Burk and secondary plot analyses. Activation free energies (Delta G#) and catalytic efficiencies were quantitatively correlated with solvatochromic scales, identifying pi* as the most relevant descriptor under activating conditions. These results advance molecular-level understanding of enzyme behavior in nonconventional media and provide practical guidelines for solvent selection in chemo-enzymatic catalysis. Solvent polarity, assessed via log P values, revealed a direct correlation with enzyme activity, where solvents with log P between 1.5 and 3.0 favored higher catalytic efficiency. Dielectric constants influenced electrostatic interactions, with optimal enzyme performance observed in solvents possessing dielectric constants around 20. Solvatochromic parameters further illustrated how hydrogen-bond donor and acceptor properties modulate the enzyme's active site configuration, thereby affecting catalytic outcomes. These findings suggest that solvent environments play a critical role in shaping both kinetic and thermodynamic profiles of Bacillus clausii protease, offering insights for optimizing biocatalytic processes in nonaqueous media.To our knowledge, this is the first study to systematically correlate kinetic and thermodynamic parameters with these solvent descriptors in protease catalysis. The findings provide fundamental insights for biotechnological applications involving enzyme-solvent interactions, paving the way for rational solvent selection in industrial biocatalysis.