Gene-Editing Technologies and Applications in Legumes: Progress, Evolution, and Future Prospects


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Baloglu M. C., Celik Altunoglu Y., Baloglu P., Yildiz A. B., Türkölmez N., Özden Çiftçi Y.

Frontiers in Genetics, cilt.13, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 13
  • Basım Tarihi: 2022
  • Doi Numarası: 10.3389/fgene.2022.859437
  • Dergi Adı: Frontiers in Genetics
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals
  • Anahtar Kelimeler: genome-editing methods, CRISPR, Cas9, TALEN, ZFN, legumes, AGROBACTERIUM-MEDIATED TRANSFORMATION, ZINC-FINGER NUCLEASES, ARACHIS-HYPOGAEA L., TARGETED MUTAGENESIS, CRISPR/CAS9 SYSTEM, PROVIDES INSIGHT, REFERENCE GENOME, ACID DESATURASE, PISUM-SATIVUM, PLANT
  • Kocaeli Üniversitesi Adresli: Hayır

Özet

Copyright © 2022 Baloglu, Celik Altunoglu, Baloglu, Yildiz, Türkölmez and Özden Çiftçi.Legumes are rich in protein and phytochemicals and have provided a healthy diet for human beings for thousands of years. In recognition of the important role they play in human nutrition and agricultural production, the researchers have made great efforts to gain new genetic traits in legumes such as yield, stress tolerance, and nutritional quality. In recent years, the significant increase in genomic resources for legume plants has prepared the groundwork for applying cutting-edge breeding technologies, such as transgenic technologies, genome editing, and genomic selection for crop improvement. In addition to the different genome editing technologies including the CRISPR/Cas9-based genome editing system, this review article discusses the recent advances in plant-specific gene-editing methods, as well as problems and potential benefits associated with the improvement of legume crops with important agronomic properties. The genome editing technologies have been effectively used in different legume plants including model legumes like alfalfa and lotus, as well as crops like soybean, cowpea, and chickpea. We also discussed gene-editing methods used in legumes and the improvements of agronomic traits in model and recalcitrant legumes. Despite the immense opportunities genome editing can offer to the breeding of legumes, governmental regulatory restrictions present a major concern. In this context, the comparison of the regulatory framework of genome editing strategies in the European Union and the United States of America was also discussed. Gene-editing technologies have opened up new possibilities for the improvement of significant agronomic traits in legume breeding.