Akademik Veri Yönetim Sistemi
1st International Conference on Frontiers in Academic Research, Konya, Türkiye, 18 - 21 Şubat 2023, ss.551-555
This study describes the production and purification of laccase from Trametes versicolor isolate. The activated fungus was grown in 50 mL YpSs broth medium for 3 days at 30 °C and 120 rpm shaking speed. Then, pellets were transferred to the laccase production medium including 5 g/L glucose, 1 g/L yeast extract, 1 g/L beef extract, 0.2 g/L pepton, 1 g/L KH2PO4, 0.5 g/L MgSO4, 0.1 g/L CuSO4, 3 g/L (NH4)2SO4, 1 g/L CaCl2, and 1 g/L Na2HPO4. The highest enzyme activity was found after the sixth day of cell growth. For purification of laccase from crude extract, three-phase partitioning was used for the first time. The enzyme was purified by 9.5-fold with an enzyme activity recovery of 93% from the TPP system prepared at pH 7.0 with a ratio of 1:1 crude extract:t-butanol and containing 30% (w/w) ammonium sulphate. As a result, it is shown that the laccase enzyme from T. versicolor can be purified using three-phase systems, which are timesaving, inexpensive, and easy to use, instead of the traditional chromatography method.