In this study, several heat treatments were applied to DIN 100Cr6 steel to obtain different matrices. In the first stage of the study, solution annealing treatment was applied to the steel and cooling was carried out in various media (furnace, oil, and salt bath). In order to eliminate the stresses after transformation from austenization, a low temperature tempering treatment was applied to the quenched samples. All heat treated samples were examined using light microscopy after metallographic preparations. In the second step, 'ball-on-disc' type tribometer was used to determine the friction coefficient of the steels depending on the matrix phase. Weight loss was recorded and the friction coefficient versus distance was plotted for each steel. Worn surfaces of the steels were examined using scanning electron microscopy to characterize the wear mechanisms. It turned out that (i) pearlitic, bainitic and martensitic matrices could be obtained depending on the cooling medium, (ii) martensitic matrix had higher wear resistance based on its weight loss, (iii) abrasive and adhesive wear tracks were present on the worn surfaces of the steels.