Research Information System
Plant Cell, Tissue and Organ Culture, vol.154, no.1, pp.97-110, 2023 (SCI-Expanded, Scopus)
The effects of plant growth regulators (PGRs) on seed germination and organ development in Pancratium maritimum L. (Sea daffodil) were evaluated comparatively. Also, the callogenesis response of in vitro-generated leaf, root, and bulb explants to PGR combinations was studied within the scope of this study. The effects of 6-Benzylaminopurine (BAP), kinetin (KIN), thidiazuron (TDZ), 3-Indoleacetic acid (IAA), indole-3-butyric acid (IBA), 1-Naphthaleneacetic acid (NAA) were tested on seed germination and organ development. The explants were cultured on 2,4-Dichlorophenoxyacetic acid (2,4-D) and IBA combined with BAP and TDZ for callogenesis experiments. TDZ treatments increased seed germination (86.67 ± 5.77%), leaf number per plant (1.50 ± 0.17), and mean leaf length (5.19 ± 0.51 cm). IBA treatments enhanced rhizogenic response. Root length increased up to 3.76 ± 0.34 cm in the presence of IBA at 1.0 mg L− 1. However, NAA treatments significantly restricted root elongation. Hierarchical cluster analysis revealed that TDZ (1.0 and 2.0 mg L− 1) and IBA (0.25 and 1.0 mg L− 1) treatments exhibited similar seed germination and organ development results. The bulb explants (46.67–93.33%) were more potent for callus production than leaf (up to 23.33%) and root (up to 26.67%) explants. IBA treatments had a synergistic impact on callus induction from in vitro-generated bulb explants when paired with BAP or TDZ. The bulb calli gave a better organogenesis response to IBA than TDZ treatment at 1.0 mg L− 1. Consequently, in vitro seed germination, organ development, and callus production protocols for P. maritimum have been developed through a comprehensive evaluation of PGRs’ effects. Also, in vitro-grown plants were transferred to their natural habitat.