Synergistic anticancer effects of metformin and Achillea vermicularis Trin-loaded nanofibers on human pancreatic cancer cell line: An in vitro study

Babazade R., Polat E. B., ERTAŞ B., Ozcan G. S., Kirmaci H. K., TATAR E., ...More

EUROPEAN POLYMER JOURNAL, vol.179, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 179
  • Publication Date: 2022
  • Doi Number: 10.1016/j.eurpolymj.2022.111565
  • Journal Name: EUROPEAN POLYMER JOURNAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Chemical Abstracts Core, Communication Abstracts, INSPEC, Metadex, Civil Engineering Abstracts
  • Keywords: Pancreatic cancer, Metformin, Achillea vermicularis Trin, Nanofiber, Electrospinning, DRUG-RELEASE, ELECTROSPUN, DEGRADATION, MECHANISM, DIAMETER, GROWTH
  • Kocaeli University Affiliated: Yes

Abstract

Treatment options for pancreatic cancer are limited and not effective enough despite recent advances in chemotherapy. In this study, Metformin (Met)/Achillea vermicularis Trin. (AV)-loaded polylactic acid (PLA)/ polycaprolactone (PCL) nanofibers (NFs) were fabricated by electrospinning and their chemical, mechanical, degradation, thermal, drug release behaviour and kinetic, and morphological properties were characterized. Met/AV-loaded NFs were found more effective against insulinoma cells in vitro than Met-loaded and AV-loaded NFs. Lactate dehydrogenase (LDH) leakage was remarkably higher in Met/AV-loaded NF-treated cells (on day 1 is 50.14% and on day 7 is 46.59%) than in pure NF-treated cells. The density of live cells was lower on the surface of Met/AV-loaded NF compared to Met-loaded and AV-loaded NFs. Met/AV-loaded NF demonstrated a higher apoptosis-inducing effect on insulinoma cells than single drug-loaded NFs according to DAPI nuclear staining. The combination of Met and AV in PLA/PCL NFs could be a novel and effective therapy against pancreatic cancer via a local application.