© 2020 Refik Saydam National Public Health Agency (RSNPHA.Objective: Cervical cancer is the second most common cancer type among women all over the world. Human papilloma virus (HPV) is the major etiologic agent associated with cervical cancer. More than 200 species of HPV have been identified so far and 40 of them are known to infect the genital system. The aim of this study was to determine the presence of HPV DNA and HPV genotypes in the cervical samples sent to our laboratory for HPV DNA investigation and to investigate the cytopathological changes in HPV positive patients. Methods: A sample of 1068 cervical swabs taken from patients between the ages of 20 and 66 applied to the Obstetrics and Gynecology Clinic between 01 January 2015 and 30 January 2018 were included in the study. HPVsign® Q24 complate kit (Qiagen, Germany) was used for HPV typing with QIAamp® DNA Mini Kit PCR (Diatech Pharmocogenetics, Italy) and pyrosequencing for DNA isolation. Results: HPV DNA was detected as positive in 226 (21.1%) samples of 1068 samples. In 141(62.3%) of the HPV DNA positive specimens, high-risk types were detected alone. Among HPV DNA positive samples, HPV type 16 was found in 73(32.3%) patients alone, multiple types were found in 37 (16.3%) patients and other types were found in 116 patients. HPV DNA positivity was highest in the 20-30 age group (26.3%). Cytology positivity was found in 20 (8.8%) of the patients with HPV DNA positivity. Of these patients, 13 had LSIL (65%), 4 (20%) had HGSIL, 1 (5%) had ASCUS, 2 (10%) had HGSIL / seviks CA. Conclusion: This is the first comprehensive study conducted in our region. The rate of HPV DNA positivity detected in cervical swab specimens and the frequency of HPV type 16 were found to be somewhat higher than the rates found in other studies in our country. More extensive work is needed to determine the type of HPV DNA and HPV type prevalence and the types of HPV that should be used in our country.