© 2020, ALÖKI Kft., Budapest, Hungary.Laurus nobilis L. a member of Lauraceae family, is a beautiful medicinal and aromatic, evergreen plant. In this study shortening of in vitro germination time and the appropriate sucrose and agar concentration for this approach were investigated. Excessive sucrose concentration negatively affected germination percentage, root length, shooting percentage and shoot length in Murashige and Skoog Medium (MS). Medium without sucrose (0 g/L sucrose) having the highest germination rate (96.3%), the highest root length per plant (3.84 cm), the highest shooting rate (96.3%) and the highest shoot length per plant (1.68 cm) in all concentrations of sucrose used (0, 10, 20 and 30 g/L) after 4 weeks of culturing. Although sucrose and agar interactions exerted effects on the germination percentage, additionally all concentrations of agar used (3, 6 and 9 g/L) showed that adding excessive amounts to the growth medium was not necessary to induce germination and shooting in general. 0 mg/L sucrose and 3 g/L agar found optimum composition of the media for both rooting and shooting in all used media. This protocol will be useful for rapid and economic large-scale in vitro cultivation of Laurus nobilis to obtain aseptic seedlings.