Purification and recovery of invertase from potato tubers (Solanum tuberosum) by three phase partitioning and determination of kinetic properties of purified enzyme


DUMAN Y. , KAYA E.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.39, ss.443-448, 2014 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 39 Konu: 4
  • Basım Tarihi: 2014
  • Doi Numarası: 10.5505/tjb.2014.78949
  • Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Sayfa Sayısı: ss.443-448

Özet

Objective: Three phase partitioning (TPP) is a simple and economical purification and recovery technique for proteins and also enzymes. In this technique; proteins are separated in the interfacial or lower phase while contaminant parts of crude extract partitioning occur in the upper organic phase. In this study invertase was purified and recovered from potato by TPP.