Purification and recovery of invertase from potato tubers (Solanum tuberosum) by three phase partitioning and determination of kinetic properties of purified enzyme
TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.39, sa.4, ss.443-448, 2014 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 39 Sayı: 4
- Basım Tarihi: 2014
- Doi Numarası: 10.5505/tjb.2014.78949
- Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.443-448
- Kocaeli Üniversitesi Adresli: Evet
Özet
Objective: Three phase partitioning (TPP) is a simple and economical purification and recovery technique for proteins and also enzymes. In this technique; proteins are separated in the interfacial or lower phase while contaminant parts of crude extract partitioning occur in the upper organic phase. In this study invertase was purified and recovered from potato by TPP.